Animal Pathology

For 30 years CONVET S.L. has been offering our services to the clinical veterinaries in order to collaborate on the diagnosis of animal pathologies by placing at their disposal our specialised human team together with the innovative technologies that allow obtaining reliable and quick results.

animal pathology

For 30 years CONVET SL has offered its services to clinical veterinarians to collaborate in the diagnosis of animal pathologies.

See information

PORCINE

Analytics Catalog

Microbiology


DETERMINATION TECHNIQUE
Actinobacillus spp Plate culture
Antibiogram Kirby-Bauer
Bordetella bronchiseptica Plate culture
Brachyspira hyodisenteriae Plate culture
Brachyspira hyodisenteriae - characterisation MLVA
Semen quality Plate culture/ Biochemical tests
Clostridium difficile Plate culture
Clostridium perfringens Plate culture
Corynebacterium SPP Plate culture
Enterobacteriaceae Plate culture
Erysipelothrix Rhusiopathiae (Red Malaise) Plate culture
Escherichia coli Plate culture
Parasitic study Flotation/McMASTER
Glaesserella parasuis Plate culture/PCR
Histopathology Microscopy/Macroscopy
Fungi Plate culture
MIC (minimum inhibitory concentration) Broth microdilution
Necropsy Macroscopy
Pasteurella multocida Plate culture
Salmonella spp ISO 6579-1-2017 / ELFA
Salmonella spp - Serotyping Antisera
Seminogram Microscopy
Staphylococcus spp Plate culture
Streptococcus spp Plate culture
Trueperella pyogenes Plate culture

Immunology


DETERMINATION TECHNIQUE
Actinobacillus pleuropneumoniae (APP) ELISA
Actinobacillus pleuropneumoniae Apx IV toxin (APP ApxIV) ELISA
Actinobacillus pleuropneumoniae Serotype 1-9-11 (APP 1-9-11) ELISA
Actinobacillus pleuropneumoniae  Serotype 2 (APP 2) ELISA
Actinobacillus pleuropneumoniae Serotype 3-6-8 (APP 3-6-8) ELISA
Actinobacillus pleuropneumoniae Serotype 4-7 (APP 4-7) ELISA
Actinobacillus pleuropneumoniae Serotype 5 (APP 5) ELISA
Actinobacillus pleuropneumoniae Serotype 10 (APP 10) ELISA
Actinobacillus pleuropneumoniae Serotype 12 (APP 12) ELISA
Actinobacillus pleuropneumoniae Serotype 13 (APP13) ELISA
Aujeszky gB (ADV gB) ELISA
Aujeszky gE (ADV gE) ELISA
Aujeszky (ADV) ELISA
Chlamydia Complement fixation
Swine coronavirus (TGEV/PRCV) ELISA
Vesicular disease (SVDV) ELISA
Glaesserella parasuis ELISA
Influenza A ELISA
Lawsonia Intracellularis ELISA
Leptospira Bratislava M.A.T.
Leptospira Icterohaemorrhagiae M.A.T.
Leptospira Canícola M.A.T.
Leptospira Pomona M.A.T.
Leptospira Grippotyphosa M.A.T.
Erysipelothrix rhusiopatiae ELISA
Mycoplasma Hyopneumoniae ELISA
Porcine parvovirus (PPV) ELISA
Toxigenic Pasteurella multocida(PMT) ELISA
Porcine circovirus Type 2 IgG/IgM (PCV2) ELISA
Porcine circovirus Type 2 (PCV2) ELISA
PRRS E – Porcine reproductive and respiratory syndrome ELISA
PRRS E/A – Porcine reproductive and respiratory syndrome ELISA
PRRS Oral Fluid ELISA
Salmonella ELISA
Sarcoptes Scabiei ELISA

Molecular biology


DETERMINATION TECHNIQUE
Actinobacillus pleuropneumoniae (APP) PCR
A. pleuropneumoniae serotype: 1, 2, 3, 4, 5, 6, 7, 8, 9-11, 10, 12, 13, 14, 15, 16, 17, 18 PCR
Bordetella bronchiseptica PCR
Brachyspira hyodysenteriae PCR
Brachyspira pilosicoli PCR
Chlamydiaceae PCR
Clostridium difficile PCR
Clostridium perfringens – Toxicogenic profile (Type A o C) PCR
Porcine epidemic Diarrhea (PED) RT PCR
Erysipelothrix Rhusiopathiae PCR
Escherichia coli – Virulence factors PCR
Transmissible gastroenteritis (TGE) RT PCR
Glaesserella parasuis PCR
G. parasuis - Serotype PCR
Influenza A RT PCR
Influenza A -serotype: H1av H1hu, H1pan, H3, N1, N2 RT PCR
Lawsonia intracellularis PCR
Leptospira spp PCR
Mycoplasma hyopneumoniae PCR
Mycoplasma hyorhinis PCR
Mycoplasma hyosynoviae PCR
Mycoplasma suis PCR
Porcine parvovirus (PPV) PCR
Toxigenic Pasteurella multocida (PMT) PCR
Porcien circovirus Type 2 (PCV2) qPCR
PRRS – Porcine reproductive and respiratory syndrome RT PCR
PRRS – Sequencing
Rotavirus Type A RT PCR
Rotavirus Type C RT PCR
Streptococcus suis - Serotype PCR

Hematology


DETERMINATION TECHNIQUE
HAEMOGRAM (EDTA)
Hematocrit CE/FM
Haemoglobin CE/FM
Erythrocyte indices CE/FM
Leukocyte formula CE/FM
Platelet CE/FM
BIOCHEMISTRY (serum)
Uric acid ER
Albumin ER
ALT-GPT (Alanine Aminotransferase) ER
AST-GOT (Aspartate Aminotransferase) ER
Direct Bilirubin ER
Total Bilirubin ER
Bun-Blood urea nitrogen
Total Calcium ER
Chlorine PID
Cholesterol ER
Creatinine ER
Alkaline phosphatase ER
Phosphorus ER
GGT (Gamma Glutamyltransferase) ER
Glucose ER
Serum Iron ER
Ionogram (Chlorine, Potassium and Sodium) PID
LDH (Lactate dehydrogenase) ER
Magnesium ER
Potassium PID
Total protein ER
Albumin/Globulin ratio
Sodium PID
Triglyceride ER
Urea ER
TOXICOLOGY (serum)
Arsenic ICP-OES
Cadmium ICP-OES
Cobalt ICP-OES
Copper ICP-OES
Chromium ICP-OES
Tin ICP-OES
Lindane ICP-OES
Manganese ICP-OES
Mercury ICP-OES
Lead ICP-OES
Selenium ICP-OES
Zinc ICP-OES
HORMONES & IMMUNOASSAY (serum)
Cortisol HPLC-DAD
Progesterone ELFA
Prolactin HPLC-DAD
Testosterone ELFA

diagnostic panels

SAMPLING

  1. All samples will be sent to the laboratory perfectly packaged and identified.
  2. It is recommended to take the sample from animals that present symptoms and, if possible, that are not medicated, immediately after their death or sacrifice and with the maximum possible hygiene.
  3. Always attach an analysis request sheet , also available on the web, with as much information as possible (client, operation, contact, requested analysis).
  4. The samples must be sent in optimal maintenance conditions depending on each type of study ( refrigeration, freezing …) and send them as soon as possible ( 24-48h ).

For any other type of sample not mentioned below, we recommend contacting the laboratory.

animal feces

  • Recommendations for sampling: feces will be collected directly from the rectum, never from the ground. Absence of fungi to the naked eye.
  • The use of sterile bottles of between 100-150 ml is recommended to guarantee a minimum sample volume.
  • Recommended minimum quantity between 100 – 50 gr.
  • It is necessary to avoid the freezing of the samples, they will be kept and sent refrigerated.
  • For samples of the National Plan for the Control of Salmonella of avian origin, consult the Avian section on our website.

swabs

  • Recommendations for taking samples: Insert the swab directly into the rectum, gently rubbing it against the walls.
  • Use of swabs with specific transport medium according to the analysis technique.
    AMIES or STUART type for bacteriological analysis and liquid media or swabs without medium for Molecular Biology.
  • It is necessary to avoid the freezing of the samples, they will be kept and sent refrigerated.
  • This technique should be used only in case of difficulty in sending organs.

Corpses / Viscera

  • Recommendations for taking samples: the sending of autolytic samples will be avoided. When the shipment to the laboratory is going to be delayed or in the case of large animals, a necropsy will be carried out in the field and the viscera will be extracted, noting the lesions observed. Samples must be drawn from non-medicated animals.
  • The samples will be sent in sealed, sterile and perfectly packed containers. It is necessary to avoid mixing viscera, each organ must have its own container.
  • Send representative samples, whole, not portions and in perfect condition.
    • In the case of intestines , it is necessary to tie the ends to avoid losses or contamination.
  • Minimum quantity required: depending on the delivery possibilities, the complete viscera will be sent or samples of the injured organs will be taken.
  • It is necessary to avoid freezing the samples, they will be preserved and sent

All samples will be sent to the laboratory perfectly packaged and identified.

Download Sampling document

In HISTOPATHOLOGY , the time elapsed since death and autolysis are the main impediments for a correct evaluation of the lesions and the detection of infectious agents in the enteric cells. For example, 20-30 minutes after death there is a diffuse detachment of enterocytes from the villus tips due to autolysis, which makes the histopathological diagnosis of ETEC and I. suis impossible. At two hours postmortem, all the tissue from the middle to the tip of the villi becomes eosinophilic without distinction between cells due to autolysis. Since most samples arrive at the laboratory the day after they are collected, the exclusive shipment of intestinal samples would significantly limit, or even prevent, a proper histological evaluation. As a consequence, it is essential that, if you are not going to send live animals to the laboratory, you collect the intestinal samples right after the euthanasia of the piglets. The fragments of intestine must have 2 to 3 cm in length and proceed from 2 portions from the ileum, between 4 and 5 from the jejunum, one from the cecum, one from the proximal colon and 2 from the spiral colon and be fixed in formalin (10%) in plastic bags or containers. Mesenteric lymph nodes and liver fragments should also be sent. All these samples will be used for histopathological examination and, in some cases, also for immunohistochemistry (PED, TGE, Rotavirus) or in situ hybridization (ISH).

In addition to formalin-fixed samples, it is important to submit fresh samples for bacteriology, virology, and molecular and biological analyses . Fragments should be 10 to 15 cm in length from the ileum and jejunum and the remaining portions of the cecum and spiral colon and may be shipped in a plastic bag. Mesenteric lymph nodes and liver fragments must be sent in separate bags to avoid contamination.

The histological evaluation of the intestinal sections mentioned above should make it possible to limit the possible agents involved in the process and, in some cases, define the presence of infectious agents. For example, villous atrophy can be caused by coccidia (I. suis), Rotavirus, GETv, PEDv, Deltacoronavirus or even C. perfringens type A. If the samples belong to piglets older than five or six days, we can find sexual or asexual forms of I. suis in the cytoplasm of villus-tip enterocytes. If coccidia are not found, immunohistochemistry can be performed to detect the presence of Rotavirus or Coronavirus viral antigens in the enterocytes. In almost all cases of ETEC, histologic examination will allow detection of a myriad of coccobacillary organisms attached to the surface of the enterocyte at the base or lateral wall of the villi. C. perfringens type C will cause a transmural fibrinonecrotic and hemorrhagic lesion in a segmental distribution in the small intestine. C. difficile is the only infectious agent that will cause injury to the large intestine. Mesocolon edema is often observed. The main findings in cases of C. difficile infection are the reduction of goblet cells and a large infiltration of neutrophils in the mucosa (of the colon that, in some cases, rupture the epithelium like a volcanic eruption. C. perfringens type A is the most difficult to determine by histopathology, since it can cause villous atrophy, but the fact that this lesion is not found does not rule out this possibility. (Roberto MC Guedes)

Enteric Panel


PANEL ENTÉRICO LECHONES LACTANTES TECHNIQUE
Plate culture
Plate culture
Plate culture / PCR
Plate culture
RT PCR
RT PCR
RT PCR
RT PCR
Kirby-Bauer
Broth microdilution
Microscopy/Macroscopy
PCR
PCR
Flotation / McMASTER
TECHNIQUE
Plate culture
Plate culture
PCR
ISO 6579-1-2017 / ELFA
Plate culture
RT PCR
RT PCR
RT PCR
Kirby-Bauer
Broth microdilution
Microscopy/Macroscopy
PCR
PCR
Antisera
Flotation / McMASTER
TECHNIQUE
PCR
PCR
PCR
Microscopy/Macroscopy
Plate culture
MLVA
Broth microdilution
ELISA
TECHNIQUE
qPCR
RT PCR
RT PCR
RT PCR

Respiratory Panel


PANEL RESPIRATORIO DESTETE / CEBO TÉCNICA

Reproductive Panel


PANEL ABORTOS / INFERTILIDAD TECHNIQUE
qPCR
PCR
RT PCR
ELISA
PCR
PCR
RT PCR
RT PCR
PCR
M.A.T.
M.A.T.
M.A.T.
M.A.T.
M.A.T.
PCR
Kirby-Bauer/MIC
TECHNIQUE
Culture plate / Count
Culture plate / PCR
Culture plate
Culture plate
Culture plate
Kirby-Bauer
Broth microdilution
TECHNIQUE
Gravimetry
Microscopy
Microscopy
Microscopy
Microscopy
Microscopy
Microscopy
Microscopy
Microscopy
Microscopy
Microscopy
Microscopy
Infrared thermometer
Culture plate / Count
Cultivo en placa / Recuento
Cultivo en placa/ Identificación
TECHNIQUE
ELISA
ELISA
ELISA
ELISA
ELISA

Nerve / Joint Panel


PANEL SINTOMATOLOGÍA NERVIOSA TECHNIQUE
Plate culture / PCR
Plate culture / PCR
Kirby-Bauer
Broth microdilution
PCR
PCR
PCR
TECHNIQUE
Plate culture / PCR
Plate culture / PCR
Plate culture / PCR
Plate culture / PCR
Kirby-Bauer
Broth microdilution
PCR
PCR
BOVINE

Analytics Catalog

Microbiology


DETERMINATION TECHNIQUE
Antibiogram Kirby-Bauer
Arcanobacterium pyogenes Plate culture
Clostridium perfringens Plate culture
Escherichia coli Plate culture
Parasite study Flotation/McMASTER
Histopathology Microscopy/Macroscopy
Histophilus somni Plate culture
Mannheimia haemolytica Plate culture
MIC (minimum inhibitory concentration) Broth microdilution
Necropsy Macroscopy
Blood parasites Brightfield optical microscopy
Pasteurella multocida Plate culture
Salmonella spp ISO 6579-1-2017 / ELFA
Salmonella spp - Serotyping Antisera
Staphylococcus spp Plate culture

Immunology


DETERMINATION TECHNIQUE
Bovine viral diarrhoea Ag (BVD Ag) ELISA
Bovine viral diarrhoea (BVD) ELISA
Bovine viral diarrhoea protein p80 (BVD p80) ELISA
Chlamydia abortus ELISA
Coxierlla burnetii (Q fever) ELISA
Infectious bovine rhinotracheitis (IBR) ELISA
Infectious bovine rhinotracheitis gE (IBR gE) ELISA
Mycoplasma bovis ELISA
Neospora caninum ELISA
Paratuberculosis ELISA
Parainfluenza 3 (PI-3) ELISA
Respiratory syncytial virus (RSV) ELISA
Rotavirus Immunochromatography
Coronavirus Immunochromatography
E.COLI k99 (f5) Immunochromatography
Cryptosporidium parvum Immunochromatography

Molecular biology


DETERMINATION TECHNIQUE
Bovine viral diarrhoea (BVD) PCR
Chlamydophila abortus PCR
Coxiella burnetii (Q fever) PCR
Histophilus somni PCR
Infectious bovine rhinotracheitis (IBR) PCR
Mannheimia haemolytica PCR
Mycoplasma bovis PCR
Neospora PCR
Leptospira spp PCR
Parainfluenza 3 (PI3) PCR
Respiratory syncytial virus (RSV) PCR

Hematology


DETERMINATION TECHNIQUE
HAEMOGRAM (S.EDTA)
Hematocrit CE/FM
Haemoglobin CE/FM
Erythrocyte indices CE/FM
Leukocyte Formula CE/FM
Platelets CE/FM
BIOCHEMISTRY (serum)
Uric Acid ER
Albumin ER
ALT-GPT (Alanine Aminotransferase) ER
AST-GOT (Aspartate Aminotransferase) ER
Direct Bilirubin ER
Total Bilirubin ER
Bun-Urea Nitrogen
Total Calcium ER
Chlorine PID
Cholesterol ER
Creatinine ER
Alkaline Phosphatase ER
Phosphorus ER
GGT (Gamma Glutamyl Transpeptidase) ER
Glucose ER
Iron ER
Ionogram (Chlorine, Potassium and Sodium) PID
LDH (Lactate Dehydrogenase) ER
Magnesium ER
Potassium PID
Total Protein ER
Albumin/Globulin Ratio
Sodium PID
Triglycerides ER
Urea ER
TOXICOLOGY (serum)
Arsenic ICP-OES
Cadmium ICP-OES
Cobalt ICP-OES
Copper ICP-OES
Chromium ICP-OES
Tin ICP-OES
Lindane ICP-OES
Manganese ICP-OES
Mercury ICP-OES
Lead ICP-OES
Selenium ICP-OES
Zinc ICP-OES
HORMONES & IMMUNOASSAY (serum)
Cortisol HPLC-DAD
Progesterone ELFA
Prolactin HPLC-DAD
Testosterone ELFA

enteric panel

Enteric Panel


PANEL ENTÉRICO TECHNIQUE
Plate culture
Plate culture
Plate culture
ISO 6579-1-2017 / ELFA
Immunochromatography
Immunochromatography
Immunochromatography
Immunochromatography
Kirby-Bauer
Broth microdilution
Microscopy/Macroscopy
PCR
PCR
Flotation/McMASTER

Respiratory Panel


PANEL RESPIRATORIO TECHNIQUE
Plate culture/PCR
Plate culture/PCR
Plate culture
Plate culture
Plate culture/PCR
Plate culture
Plate culture
Kirby-Bauer
Broth microdilution
PCR / Sequencing
PCR
RT-PCR
PCR / Sequencing
PCR

Reproductive Panel


PANEL ABORTOS / INFERTILIDAD TECHNIQUE
ELFA
ISO 6579-1-2017 / ELFA
Plate culture
Kirby-Bauer
Broth microdilution
PCR
ELISA
PCR
Flotation/McMASTER
Microscopy/Macroscopy
MAT / PCR
ELISA
PCR
TECHNIQUE
Plate culture
Plate culture
Plate culture
Plate culture
Kirby-Bauer
Broth microdilution
PCR
TECHNIQUE
Complement fixation
ELISA
ELISA
ELISA
ELISA

ONA Panel


PANEL OCULAR TECHNIQUE
Plate culture
Plate culture
Plate culture
Kirby-Bauer
Broth microdilution
PCR
TECHNIQUE
ELFA
Plate culture
Kirby-Bauer
Broth microdilution
TECHNIQUE
Plate culture/PCR
Plate culture
Plate culture
Kirby-Bauer
Broth microdilution
AVIAN

Analytics Catalog

Microbiology


DETERMINATION TECHNIQUE
Antiobiogram Kirby-Baue
Avibacterium paragallinarum Plate culture
Campylobacter spp Plate culture
Chlamydia Plate culture
Clostridium perfringens Plate culture
Total Enterobacteriaceae Plate culture
Enterococcus spp Plate culture
Escherichia coli Plate culture
Parasitic study Flotation/ McMASTER
Histopathology Microscopy/Macroscopy
Listeria monocytogenes ELFA
MIC (minimum inhibitory concentration) Broth microdilution
Necropsy Macroscopy
Ornithobacterium rhinotracheale Plate culture
Pasteurella spp Plate culture
Fungal pneumonia Plate culture
Pseudomonas spp Plate culture
Salmonella spp ISO 6579-1-2017 / ELFA
Salmonella spp - Serotype Antiserum
Staphylococcus spp Plate culture

Immunology


DETERMINATION TECHNIQUE
Adenovirus group I ELISA
Infectious bronchitis ELISA
Chiken anemia virus ELISA
EDS- Egg drop syndorme ELISA
Encefalomielitis ELISA
Gumboro disease ELISA
Avian Influenza ELISA
Infectious Laringotracheitis ELISA
Avian Leukosis ELISA
Mycoplasma gallisepticum ELISA
Mycoplasma meleagridis ELISA
Mycoplasma synoviae ELISA
Newcastle ELISA
Reovirus ELISA
Reticuloendotheliosis ELISA
Salmonella enteritidis ELISA
Salmonella enteritidis/typhimurium ELISA
Other determinations (consult)

Molecular biology


DETERMINATION TECHNIQUE
Adenovirus group I
Infectious bronchitis RT PCR
Gumboro disease RT PCR
Newcastle disease RT PCR
Laryngotracheitis PCR
Mycoplasma synoviae PCR
Reovirus RT PCR
Egg drop syndrome PCR

Hematology


DETERMINATION TECHNIQUE
HEMOGRAM (S.EDTA)
CBC in chamber Manual
Haematocrit Manual
Haemoglobin Manual
Leukocyte formula Manual
Smear study Manual
BIOCHEMISTRY (serum)
Uric acid ER
Albumin ER
ALT-GPT (Alanine aminotransferase) ER
AST-GOT (Aspartate aminotransferase) ER
Direct bilirubin ER
Total bilirubin ER
Bun-nitrogen urea
Total calcium ER
Chlorine PID
Cholesterol ER
Creatinine ER
Alkaline phosphatase ER
Phosphorus ER
GGT (Gamma glutamyl transpeptidase) ER
Glucose ER
Iron ER
LDH (Lactate dehydrogenase) PID
Magnesium ER
Potassium PID
Total protein ER
Albumin/globulin ratio
Sodium PID
Triglycerides ER
Urea ER
TOXICOLOGY (serum)
Arsenic ICP-OES
Cadmium ICP-OES
Cobalt ICP-OES
Copper ICP-OES
Chromium ICP-OES
Tin ICP-OES
Lindane ICP-OES
Manganese ICP-OES
Mercury ICP-OES
Lead ICP-OES
Selenium ICP-OES
Zinc ICP-OES

Control Plans

National Salmonella Control Plan


DETERMINATION TECHNIQUE
Salmonella spp ISO 6579-1-2017 / ELFA
Salmonella spp - serotype Antiserum
S. enteritidis - vaccine strain/field strain differentiation PCR

Campylobacter control plan in broiler carcasses


DETERMINATION TECHNIQUE
Campylobacter spp - Counts Plate Culture / Automated NMP
Campylobacter spp ELFA
Campylobacter jejuni PCR
Campylobacter coli PCR

Our main goal is the detection and prevention; this is the reason why we cooperate with companies in the sector under exhaustive biosecurity plans that guarantee the proper hygiene and health conditions of our agricultural companies.

The study of the clinical case starts with an anamnesis of the farm to structure the clinical history. We should not forget that this history becomes a tool that will provide us with lots of relevant information for the final diagnosis of the pathology being studied.

The departments of Immunology, Microbiology, Parasitology, Virology, Molecular Biology and Haematology propose a wide range of analytical determinations to carry out multiple diagnostic profiles by means of population and individual monitoring. We work for the detection of pathologies related to animal husbandries and the control of animals addressed to export.

See sections
PORCINE

Analytics Catalog

Microbiology


DETERMINATION TECHNIQUE
Actinobacillus spp Plate culture
Antibiogram Kirby-Bauer
Bordetella bronchiseptica Plate culture
Brachyspira hyodisenteriae Plate culture
Brachyspira hyodisenteriae - characterisation MLVA
Semen quality Plate culture/ Biochemical tests
Clostridium difficile Plate culture
Clostridium perfringens Plate culture
Corynebacterium SPP Plate culture
Enterobacteriaceae Plate culture
Erysipelothrix Rhusiopathiae (Red Malaise) Plate culture
Escherichia coli Plate culture
Parasitic study Flotation/McMASTER
Glaesserella parasuis Plate culture/PCR
Histopathology Microscopy/Macroscopy
Fungi Plate culture
MIC (minimum inhibitory concentration) Broth microdilution
Necropsy Macroscopy
Pasteurella multocida Plate culture
Salmonella spp ISO 6579-1-2017 / ELFA
Salmonella spp - Serotyping Antisera
Seminogram Microscopy
Staphylococcus spp Plate culture
Streptococcus spp Plate culture
Trueperella pyogenes Plate culture

Immunology


DETERMINATION TECHNIQUE
Actinobacillus pleuropneumoniae (APP) ELISA
Actinobacillus pleuropneumoniae Apx IV toxin (APP ApxIV) ELISA
Actinobacillus pleuropneumoniae Serotype 1-9-11 (APP 1-9-11) ELISA
Actinobacillus pleuropneumoniae  Serotype 2 (APP 2) ELISA
Actinobacillus pleuropneumoniae Serotype 3-6-8 (APP 3-6-8) ELISA
Actinobacillus pleuropneumoniae Serotype 4-7 (APP 4-7) ELISA
Actinobacillus pleuropneumoniae Serotype 5 (APP 5) ELISA
Actinobacillus pleuropneumoniae Serotype 10 (APP 10) ELISA
Actinobacillus pleuropneumoniae Serotype 12 (APP 12) ELISA
Actinobacillus pleuropneumoniae Serotype 13 (APP13) ELISA
Aujeszky gB (ADV gB) ELISA
Aujeszky gE (ADV gE) ELISA
Aujeszky (ADV) ELISA
Chlamydia Complement fixation
Swine coronavirus (TGEV/PRCV) ELISA
Vesicular disease (SVDV) ELISA
Glaesserella parasuis ELISA
Influenza A ELISA
Lawsonia Intracellularis ELISA
Leptospira Bratislava M.A.T.
Leptospira Icterohaemorrhagiae M.A.T.
Leptospira Canícola M.A.T.
Leptospira Pomona M.A.T.
Leptospira Grippotyphosa M.A.T.
Erysipelothrix rhusiopatiae ELISA
Mycoplasma Hyopneumoniae ELISA
Porcine parvovirus (PPV) ELISA
Toxigenic Pasteurella multocida(PMT) ELISA
Porcine circovirus Type 2 IgG/IgM (PCV2) ELISA
Porcine circovirus Type 2 (PCV2) ELISA
PRRS E – Porcine reproductive and respiratory syndrome ELISA
PRRS E/A – Porcine reproductive and respiratory syndrome ELISA
PRRS Oral Fluid ELISA
Salmonella ELISA
Sarcoptes Scabiei ELISA

Molecular biology


DETERMINATION TECHNIQUE
Actinobacillus pleuropneumoniae (APP) PCR
A. pleuropneumoniae serotype: 1, 2, 3, 4, 5, 6, 7, 8, 9-11, 10, 12, 13, 14, 15, 16, 17, 18 PCR
Bordetella bronchiseptica PCR
Brachyspira hyodysenteriae PCR
Brachyspira pilosicoli PCR
Chlamydiaceae PCR
Clostridium difficile PCR
Clostridium perfringens – Toxicogenic profile (Type A o C) PCR
Porcine epidemic Diarrhea (PED) RT PCR
Erysipelothrix Rhusiopathiae PCR
Escherichia coli – Virulence factors PCR
Transmissible gastroenteritis (TGE) RT PCR
Glaesserella parasuis PCR
G. parasuis - Serotype PCR
Influenza A RT PCR
Influenza A -serotype: H1av H1hu, H1pan, H3, N1, N2 RT PCR
Lawsonia intracellularis PCR
Leptospira spp PCR
Mycoplasma hyopneumoniae PCR
Mycoplasma hyorhinis PCR
Mycoplasma hyosynoviae PCR
Mycoplasma suis PCR
Porcine parvovirus (PPV) PCR
Toxigenic Pasteurella multocida (PMT) PCR
Porcien circovirus Type 2 (PCV2) qPCR
PRRS – Porcine reproductive and respiratory syndrome RT PCR
PRRS – Sequencing
Rotavirus Type A RT PCR
Rotavirus Type C RT PCR
Streptococcus suis - Serotype PCR

Hematology


DETERMINATION TECHNIQUE
HAEMOGRAM (EDTA)
Hematocrit CE/FM
Haemoglobin CE/FM
Erythrocyte indices CE/FM
Leukocyte formula CE/FM
Platelet CE/FM
BIOCHEMISTRY (serum)
Uric acid ER
Albumin ER
ALT-GPT (Alanine Aminotransferase) ER
AST-GOT (Aspartate Aminotransferase) ER
Direct Bilirubin ER
Total Bilirubin ER
Bun-Blood urea nitrogen
Total Calcium ER
Chlorine PID
Cholesterol ER
Creatinine ER
Alkaline phosphatase ER
Phosphorus ER
GGT (Gamma Glutamyltransferase) ER
Glucose ER
Serum Iron ER
Ionogram (Chlorine, Potassium and Sodium) PID
LDH (Lactate dehydrogenase) ER
Magnesium ER
Potassium PID
Total protein ER
Albumin/Globulin ratio
Sodium PID
Triglyceride ER
Urea ER
TOXICOLOGY (serum)
Arsenic ICP-OES
Cadmium ICP-OES
Cobalt ICP-OES
Copper ICP-OES
Chromium ICP-OES
Tin ICP-OES
Lindane ICP-OES
Manganese ICP-OES
Mercury ICP-OES
Lead ICP-OES
Selenium ICP-OES
Zinc ICP-OES
HORMONES & IMMUNOASSAY (serum)
Cortisol HPLC-DAD
Progesterone ELFA
Prolactin HPLC-DAD
Testosterone ELFA

diagnostic panels

SAMPLING

  1. All samples will be sent to the laboratory perfectly packaged and identified.
  2. It is recommended to take the sample from animals that present symptoms and, if possible, that are not medicated, immediately after their death or sacrifice and with the maximum possible hygiene.
  3. Always attach an analysis request sheet , also available on the web, with as much information as possible (client, operation, contact, requested analysis).
  4. The samples must be sent in optimal maintenance conditions depending on each type of study ( refrigeration, freezing …) and send them as soon as possible ( 24-48h ).

For any other type of sample not mentioned below, we recommend contacting the laboratory.

animal feces

  • Recommendations for sampling: feces will be collected directly from the rectum, never from the ground. Absence of fungi to the naked eye.
  • The use of sterile bottles of between 100-150 ml is recommended to guarantee a minimum sample volume.
  • Recommended minimum quantity between 100 – 50 gr.
  • It is necessary to avoid the freezing of the samples, they will be kept and sent refrigerated.
  • For samples of the National Plan for the Control of Salmonella of avian origin, consult the Avian section on our website.

swabs

  • Recommendations for taking samples: Insert the swab directly into the rectum, gently rubbing it against the walls.
  • Use of swabs with specific transport medium according to the analysis technique.
    AMIES or STUART type for bacteriological analysis and liquid media or swabs without medium for Molecular Biology.
  • It is necessary to avoid the freezing of the samples, they will be kept and sent refrigerated.
  • This technique should be used only in case of difficulty in sending organs.

Corpses / Viscera

  • Recommendations for taking samples: the sending of autolytic samples will be avoided. When the shipment to the laboratory is going to be delayed or in the case of large animals, a necropsy will be carried out in the field and the viscera will be extracted, noting the lesions observed. Samples must be drawn from non-medicated animals.
  • The samples will be sent in sealed, sterile and perfectly packed containers. It is necessary to avoid mixing viscera, each organ must have its own container.
  • Send representative samples, whole, not portions and in perfect condition.
    • In the case of intestines , it is necessary to tie the ends to avoid losses or contamination.
  • Minimum quantity required: depending on the delivery possibilities, the complete viscera will be sent or samples of the injured organs will be taken.
  • It is necessary to avoid freezing the samples, they will be preserved and sent

All samples will be sent to the laboratory perfectly packaged and identified.

Download Sampling document

In HISTOPATHOLOGY , the time elapsed since death and autolysis are the main impediments for a correct evaluation of the lesions and the detection of infectious agents in the enteric cells. For example, 20-30 minutes after death there is a diffuse detachment of enterocytes from the villus tips due to autolysis, which makes the histopathological diagnosis of ETEC and I. suis impossible. At two hours postmortem, all the tissue from the middle to the tip of the villi becomes eosinophilic without distinction between cells due to autolysis. Since most samples arrive at the laboratory the day after they are collected, the exclusive shipment of intestinal samples would significantly limit, or even prevent, a proper histological evaluation. As a consequence, it is essential that, if you are not going to send live animals to the laboratory, you collect the intestinal samples right after the euthanasia of the piglets. The fragments of intestine must have 2 to 3 cm in length and proceed from 2 portions from the ileum, between 4 and 5 from the jejunum, one from the cecum, one from the proximal colon and 2 from the spiral colon and be fixed in formalin (10%) in plastic bags or containers. Mesenteric lymph nodes and liver fragments should also be sent. All these samples will be used for histopathological examination and, in some cases, also for immunohistochemistry (PED, TGE, Rotavirus) or in situ hybridization (ISH).

In addition to formalin-fixed samples, it is important to submit fresh samples for bacteriology, virology, and molecular and biological analyses . Fragments should be 10 to 15 cm in length from the ileum and jejunum and the remaining portions of the cecum and spiral colon and may be shipped in a plastic bag. Mesenteric lymph nodes and liver fragments must be sent in separate bags to avoid contamination.

The histological evaluation of the intestinal sections mentioned above should make it possible to limit the possible agents involved in the process and, in some cases, define the presence of infectious agents. For example, villous atrophy can be caused by coccidia (I. suis), Rotavirus, GETv, PEDv, Deltacoronavirus or even C. perfringens type A. If the samples belong to piglets older than five or six days, we can find sexual or asexual forms of I. suis in the cytoplasm of villus-tip enterocytes. If coccidia are not found, immunohistochemistry can be performed to detect the presence of Rotavirus or Coronavirus viral antigens in the enterocytes. In almost all cases of ETEC, histologic examination will allow detection of a myriad of coccobacillary organisms attached to the surface of the enterocyte at the base or lateral wall of the villi. C. perfringens type C will cause a transmural fibrinonecrotic and hemorrhagic lesion in a segmental distribution in the small intestine. C. difficile is the only infectious agent that will cause injury to the large intestine. Mesocolon edema is often observed. The main findings in cases of C. difficile infection are the reduction of goblet cells and a large infiltration of neutrophils in the mucosa (of the colon that, in some cases, rupture the epithelium like a volcanic eruption. C. perfringens type A is the most difficult to determine by histopathology, since it can cause villous atrophy, but the fact that this lesion is not found does not rule out this possibility. (Roberto MC Guedes)

Enteric Panel


PANEL ENTÉRICO LECHONES LACTANTES TECHNIQUE
Plate culture
Plate culture
Plate culture / PCR
Plate culture
RT PCR
RT PCR
RT PCR
RT PCR
Kirby-Bauer
Broth microdilution
Microscopy/Macroscopy
PCR
PCR
Flotation / McMASTER
TECHNIQUE
Plate culture
Plate culture
PCR
ISO 6579-1-2017 / ELFA
Plate culture
RT PCR
RT PCR
RT PCR
Kirby-Bauer
Broth microdilution
Microscopy/Macroscopy
PCR
PCR
Antisera
Flotation / McMASTER
TECHNIQUE
PCR
PCR
PCR
Microscopy/Macroscopy
Plate culture
MLVA
Broth microdilution
ELISA
TECHNIQUE
qPCR
RT PCR
RT PCR
RT PCR

Respiratory Panel


PANEL RESPIRATORIO DESTETE / CEBO TÉCNICA

Reproductive Panel


PANEL ABORTOS / INFERTILIDAD TECHNIQUE
qPCR
PCR
RT PCR
ELISA
PCR
PCR
RT PCR
RT PCR
PCR
M.A.T.
M.A.T.
M.A.T.
M.A.T.
M.A.T.
PCR
Kirby-Bauer/MIC
TECHNIQUE
Culture plate / Count
Culture plate / PCR
Culture plate
Culture plate
Culture plate
Kirby-Bauer
Broth microdilution
TECHNIQUE
Gravimetry
Microscopy
Microscopy
Microscopy
Microscopy
Microscopy
Microscopy
Microscopy
Microscopy
Microscopy
Microscopy
Microscopy
Infrared thermometer
Culture plate / Count
Cultivo en placa / Recuento
Cultivo en placa/ Identificación
TECHNIQUE
ELISA
ELISA
ELISA
ELISA
ELISA